Ion Mobility

Lipidomic consequences of phospholipid synthesis defects in Escherichia coli by HILIC-ion mobility-mass spectrometry

Our understanding of phospholipid biosynthesis in Gram-positive and Gram-negative bacteria is derived from the prototypical Gram-negative organism *Escherichia coli*. The inner and outer membranes of *E. coli* are largely composed of …

Characterization of the mechanisms of daptomycin resistance among Gram-positive bacterial pathogens by multidimensional lipidomics

Previous work suggests that altered lipid metabolism may be associated with daptomycin resistance in Gram-positive pathogens, but lipidomic changes underlying resistance are not fully understood. We performed untargeted lipidomics by using …

Large-scale structural characterization of drug and drug-like compounds by high-throughput ion mobility-mass spectrometry

Ion mobility-mass spectrometry (IM-MS) can provide orthogonal information, *i.e.*, *m/z* and collision cross section (CCS), for the identification of drugs and drug metabolites. However, only a small number of CCS values are available for drugs, …

Assessment of altered lipid homeostasis by HILIC-ion mobility-mass spectrometry-based lipidomics

Ion mobility-mass spectrometry (IM-MS) has proven to be a highly informative technique for the characterization of lipids from cells and tissues. We report the combination of hydrophilic-interaction liquid chromatography (HILIC) with traveling-wave …

Evaluation of collision cross section calibrants for structural analysis of lipids by traveling wave ion mobility-mass spectrometry

Collision cross section (CCS) measurement of lipids using traveling wave ion mobility-mass spectrometry (TWIM-MS) is of high interest to the lipidomics field. However, currently available calibrants for CCS measurement using TWIM are predominantly …

Structural mass spectrometry of tissue extracts to distinguish cancerous and non-cancerous breast diseases

Aberrant metabolism in breast cancer tumors has been widely studied by both targeted and untargeted analyses to characterize the affected metabolic pathways. In this work, we utilize ultra-performance liquid chromatography (UPLC) in tandem with ion …

Biomolecular signatures of diabetic wound healing by structural mass spectrometry

Wound fluid is a complex biological sample containing byproducts associated with the wound repair process. Contemporary techniques, such as immunoblotting and enzyme immunoassays, require extensive sample manipulation and do not permit the …